Gene Splicing in laboratory

Gene splicing, Genetic engineering, recombinant DNA technology,genetic modification/manipulation (GM) is process of cutting a gene from one organism and pasting it into the DNA of another so that a characteristic can be transferred from one plant or animal to another

Restriction enzymes can be used to cut the DNA at different places making the DNA sticky ends so that it can be pasted into DNA from another organism

Splicing a gene into a plasmid
In this activity, you can cut out a gene from a chromosome of one organism and paste it into a plasmid.

To do this successfully:
Be careful not to cut up the gene you are working with

Cuts the DNa with jagged ends, not blunt ones, so that it can be pasted into the plasmid,

Make sure that cut ends of the plasmid match the cut ends of the DNA


The Splicing starts with identifying the correct Restriction enzyme for the Gene, if u choose wrong restriction enzyme it may cut Gene into half rather than isolating the whole gene, So care should be taken in identifying the restriction enzyme which isolates the whole gene (in this case HindIII),

After isolating gene we should cut Plasmid with corresponding restriction enzyme (HindIII) so that gene of interest can be inserted into the plasmid.

After inserting the gene we need to seal the plasmid with Ligase enzyme to achieve a recombinant DNA.


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